Biosafety in Microbiological and Biomedical Laboratories by Jonathan Y. Richmond, Robert W. McKinney

By Jonathan Y. Richmond, Robert W. McKinney

Describes the mixtures of normal & distinct microbiological practices, defense equip., & amenities that are urged for paintings with numerous infectious brokers in a number of labs. This revision takes into consideration such concerns as international problem approximately rising & reemerging infectious ailments; elevated curiosity in prion ailments with the id of BSE in England; the incidence of numerous lab-associated infections; elevated situation concerning the nationwide & intl. move of infectious microorganisms; & starting to be matters approximately bioterrorism, & the elevated safeguard wishes of our microbiological labs.

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Locate biological safety cabinets away from doors, from windows that can be opened, from heavily traveled laboratory areas, and from other po tentially disruptive e quipm ent so as to maintain the biological safety cabinets’ air flow param eters for containm ent. 8. An eyew ash sta tion is readily ava ilable. 9. Illumination is adequate for all activities, avoiding reflections and glare that could imped e vision. 10. There are no spe cific ventilation requirements. Ho wever, planning of new facilities should consider mechanical ventila tion s ystem s tha t prov ide an inwa rd flow of air without rec irculation to s paces outside o f the labora tory.

A biohazard sign must be posted on the entrance to the laborator y when etiolo gic agen ts are in us e. Appro priate information to be posted includes the agent(s) in use, the biosafety level, the required immunizations, the investigator’s name and telephone number, any personal 22 Laboratory Biosafety Level Criteria – Biosafety Level 2 prote ctive e quipm ent th at m ust b e wo rn in th e labo rator y, and an y proced ures req uired for e xiting the labo ratory. 4. , hep atitis B vacc ine or TB skin tes ting).

3. Each la boratory c ontains a sink for h andwa shing. 4. The labor atory is desig ned so th at it ca n be e asily cleaned. Carpets and rugs in laboratories are inappropriate. 5. Bench tops are imper vious to w ater and are resis tant to moderate heat and the organic solvents, acids, alkalis, and chemicals used to decontaminate the work surfaces and eq uipm ent. 6. Laboratory furniture is capable of supporting anticipated loading and uses. Spaces between benches, cabinets, and equipment are accessible for cleaning.

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