By H. Jacquet, R. P. Langlands
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Extra info for Automorphic Forms on GL(2)
Close the line (stopcock 5, Fig. 1) and chill the reservoir in a dry ice-ethanol Dewar flask for about 5-10 min. Do not use dry ice-acetone, because acetone is much more flammable. Fluorocarbons (such as Kel-F) have a low thermal conductivity and require some time to cool down; once cold they require some time to warm up. Open the HF cylinder valve and carefully open stopcock 1 (Fig. 1), so that HF distills into the reservoir. When a good rate of HF distillation is obtained, the manometer will usually show a vacuum of about 20 torr.
Distill 10 ml of HF into the cleavage vessel and perform a standard high HF cleavage, as above.
289 Copyright © 1997 by AcademicPress All rights of reproductionin any form reserved. 00 [31 CLEAVAGEFOLLOWINGBOC-BASEDSPPS 29 Acknowledgments This work was supported (in part) by the Australian Research Grants Council. We thank Stephen Kent for support and encouragement in the continuing development of more effective SPPS chemistry.  C l e a v a g e M e t h o d s F o l l o w i n g B o c - B a s e d Peptide Synthesis Solid-Phase By JOHN M. STEWART Introduction In solid-phase peptide synthesis (SPPS) using Boc (tert-butyloxycarbonyl) amino acids, peptide chains are linked to the resin supports as benzyl esters or by bonds of similar chemical stability, and side-chain functional groups are protected by groups of similar reactivity.